Immunofluorescence staining of HAI-2-His in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS?blocked with 10% serum, and incubated with HAI-2 Polyclonal Antibody (Product # PA5-81040, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.